GROWTH AND SERUM CONCENTRATION RESPONSE CURVES OF DERMAL

FIBROBLASTS OBTAINED FROM NORMAL INDIVIDUALS AND FROM

INDIVIDUALS WITH ADENOMATOSIS OF THE COLON AND RECTUM*

Copyright © 1988 by Ernest M. Trionfo

TABLE OF CONTENTS

I. INTRODUCTION

II. REVIEW OF LITERATURE

III. MATERIALS AND METHODS

  A. Human Material

  B. Preparation of Media and Solutions

  C. Culture Conditions and Subculture Procedures

  D. Procedures

     1. Growth Measurements

     2. Determination of the Effect of Cell Density on Growth Rate

IV. RESULTS

  A. Growth Measurements

    1. Serum Requirement

    2. Effect of Culture Age on Serum Requirement

    3. Growth Curves

  B. LIST OF TABLES

  C. LIST OF FIGURES

V. DISCUSSION

VI. SUMMARY

VII. APPENDIX

VIII. LITERATURE CITED

*A Thesis Submitted to the Faculty of the College of Sciencein Partial Fulfillment of the Requirements for the Degree of Master of Science: Florida Atlantic University, Boca Raton, Florida, April 1988.

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